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The effects of alanine-scanning mutations on NFAT2 activity in vivo. Sequence of the NFAT2 RIR, with positions mutated to alanine denoted below. To the right of the sequences are shown the transcriptional activity of wild-type and mutant NFAT2-RHR proteins in the cooperative ARRE2 reporter assay, and (for selected examples) in the NFAT-AD reporter assay. The standard error in the measurements of transcriptional activity assays are estimated at ± 25%.
