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. 1997 May 13;94(10):4925–4930. doi: 10.1073/pnas.94.10.4925

Figure 1.

Figure 1

TLC (ascending) of steroid transformations in HeLa cells infected with a vaccinia-Cyp7b recombinant (VV-Cyp7b). (a) Products generated by incubating control vaccinia-infected cell extracts (v); or extracts of cells infected by VV expressing Cyp7b (7b), with steroids 25-hydroxycholesterol (25OHC), pregnenolone (Preg), DHEA, progesterone (Prog), corticosterone (B), cortisol (F), testosterone (Test), estradiol (E2), and 5α,androstane-3β,17β-diol (And); lateral arrowheads indicate the origin. (b) Products obtained with no extract control (o), control vaccinia-infected cells (v), VV-Cyp7b infected cells (7b), rat brain extract (Br), and rat liver extract (Li). (c) Products generated by 0, v, and 7b cell preparations (abbreviations as above) using intact live (vaccinia-infected) cells (ic), purified microsomes (m), or by purified microsomes from VV-Cyp7b infected cells incubated in the absence of NADPH (-N) or with 1 or 10 μM clotrimazole (1C, 10C).