Figure 3.

Release of radioactivity from [7α-³H]pregnenolone by Cyp7b and brain extracts. The set of results presented is representative of three independent sets of experiments performed under slightly different conditions but which yielded comparable values. Samples were buffer only (column a); control vaccinia (v) extract (column b); VV-Cyp7b extract (7b) (column c); 10, 20, and 50 μl rat brain (Br) extract (columns d–f); and 10, 20, and 50 μl rat liver (Li) extract (columns g–i). For reaction conditions see Experimental Procedures.