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Cell-free conversion of 35S-PrPC into PK-resistant forms. PrPSc from sheep with different PrP genotypes were pretreated in 2.5 M Gdn·HCl, except for reactions in lanes 10 and 11 in which PrPSc was denatured in 6 M Gdn·HCl. After incubation for 5 days at 37°C (1 M Gdn·HCl), the samples were PK-digested and analyzed by 15% SDS/PAGE. Molecular mass markers (kDa) are indicated at the left.
