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RT-PCR analysis of FGF-2 and FGF-AS mRNA expression in tissues isolated from embryonic (day 17 postfertilization) (A); neonatal (postnatal day 12) (B); and adult (day 120) (C). Glyceraldehyde phosphate dehydrogenase (GPD) mRNA levels were determined as a loading control. Control reactions (no RT), included for each reaction, produced no product (not shown). Lanes: 1, brain; 2, heart; 3, kidney; 4, liver.
