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RIN1 binding to SH3 domains. Labeled RIN1 peptide was incubated with purified GST (control) or GST fusion proteins with the SH3 domains of PI3 kinase, Src or c-Abl [with or without competitor (cp) or random (rp) peptides]. The mixture was then bound to glutathione Sepharose beads, washed extensively, and counted.
