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Immunoblot and methylation pattern of Pho81Wr−, Δ35, and transformant membranes. Shown are immunoblot with HC23 antibody (A) and methylation (B) fluorograms of membrane proteins from the following: a, Pho81Wr−; b, Pho81Wr− × pPR73N; c, Pho81Wr− × pPR5; d, Δ35; e, Δ35 × pPR73N; and f, Δ35 × pPR5.
