Abstract
Neurospora crassa sl, a mutant that lacks a rigid cell wall, exhibits transport systems for glucose similar to those of wild-type strain 1A. When the orgnism is grown in a medium containing 50 mM glucose as the carbon source, glucose is transported primarily by a glucose-facilitated diffusion system (GluI). When it is grown in a medium with little or no glucose present, a glucose active transport system (Glu II) is expressed. Both of these systems are similar kinetically to those in the wild type. Significant differences do exist between strains sl and 1A with respect to genetic regulation of the glucose active transport system.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLELAND W. W. Computer programmes for processing enzyme kinetic data. Nature. 1963 May 4;198:463–465. doi: 10.1038/198463a0. [DOI] [PubMed] [Google Scholar]
- Kaback H. R. Active transport in bacterial cytoplasmic membrane vesicles. Symp Soc Exp Biol. 1973;27:145–174. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
- Scarborough G. A. Isolation and characterization of Neurospora crassa plasma membranes. J Biol Chem. 1975 Feb 10;250(3):1106–1111. [PubMed] [Google Scholar]
- Scarborough G. A., Schulte T. H. An improved method for the production of Neurospora crassa spheroplasts and a new method for measuring transport in fragile cells. Anal Biochem. 1974 Oct;61(2):441–447. doi: 10.1016/0003-2697(74)90410-2. [DOI] [PubMed] [Google Scholar]
- Scarborough G. A. Sugar transport in Neurospora crassa. 3. An inositol requirement for the function of the glucose active transport system. Biochem Biophys Res Commun. 1971 Jun 4;43(5):968–975. doi: 10.1016/0006-291x(71)90557-2. [DOI] [PubMed] [Google Scholar]
- Scarborough G. A. Sugar transport in Neurospora crassa. II. A second glucose transport system. J Biol Chem. 1970 Aug 10;245(15):3985–3987. [PubMed] [Google Scholar]
- Scarborough G. A. Sugar transport in Neurospora crassa. J Biol Chem. 1970 Apr 10;245(7):1694–1698. [PubMed] [Google Scholar]
- Scarborough G. A. Transport in Neurospora. Int Rev Cytol. 1973;34:103–122. doi: 10.1016/s0074-7696(08)61935-x. [DOI] [PubMed] [Google Scholar]
- Schneider R. P., Wiley W. R. Regulation of sugar transport in Neurospora crassa. J Bacteriol. 1971 May;106(2):487–492. doi: 10.1128/jb.106.2.487-492.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]