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. 1997 May 13;94(10):4982–4987. doi: 10.1073/pnas.94.10.4982

Figure 2.

Figure 2

In vitro run-off transcription. (A) Transcription from homoduplex templates. RNA products generated from Phag(+UP) (lane 1), Phag(-UP) (lane 2), or both templates competing (lanes 3–6). Reactions contained NaCl at 100 mM (lanes 1, 2, and 4), 50 mM (lane 3), 150 mM (lane 5), or 200 mM (lane 6). (B) Transcription from competing +UP and −UP wtNT bubble templates. RNA products were generated by core (lanes 1–4) or σD-holoenzyme (lanes 5–8) in transcription reactions with NaCl at 100 mM (lanes 1 and 5), 150 mM (lanes 2 and 6), 200 mM (lanes 3 and 7), or 250 mM (lanes 4 and 8). Forward and reverse (R) transcripts derived from the +UP templates migrate more slowly than those from the −UP templates. (C) Transcription from competing +UP and −UP wtT bubble templates. RNA products were generated by core (lanes 1–6) or σD-holoenzyme (lanes 7–11) in transcription reactions with NaCl at 100 mM (lanes 1 and 7), 150 mM (lanes 2 and 8), 200 mM (lanes 3 and 9), 250 mM (lanes 4 and 10), 300 mM (lanes 5 and 11), or 350 mM (lane 6).