Figure 1.

Characterization of a T cell hybridoma lacking 5α-reductase activities. The 312.13 T cell hybridoma cells and PC3 human prostate cancer cells were incubated with 10 nM [³H]T for 48 hr. Steroid metabolites were extracted sequentially with ether and methanol and then dissolved in HPLC mobile-phase solvent (50% acetonitrile/50% water). Samples were passed through a C₁₈ HPLC column for separation. HPLC profiles of 312.13- and PC3-CM lipophilic fractions are shown as a representative of two experiments. [³H]Steroid standard was run prior to and after experimental samples for calibration and identification. The standards, in order of hydrophobicity, are 17β-estradiol (E), T, androstenedione (A), and DHT.