Figure 2.

Differential display profiles of 312.13 mRNA showing a potential androgen target fragment. The 312.13 cells were treated with 10% CDFBS (C, control) only or further supplemented with 10 nM T or 10 nM DHT for 1, 2, and 8 hr. Total RNA preparations were subjected to DDPCR. A partial differential display profile done with primers H-T₁₁G and H-AP12 is shown. Open arrowhead, TDD5 DNA bands; solid arrowhead, common bands used for normalization in quantification. Profile shown is representative of three experiments.