Figure 3.

Northern blot confirmation of differential expression of TDD5 mRNA. The 312.13 cells were treated parallel to those for DDPCR. Total RNA (25 μg per sample per lane) was resolved by electrophoresis in a formaldehyde gel, transferred to a nylon membrane, and probed with a 288-bp differential display TDD5 DNA fragment. (A) The 312.13 cells were treated with 10% CDFBS only (C, control) or further supplemented with 17β-estradiol (E₂), T, or DHT for 1, 2, and 8 hr. A representative Northern blot autoradiogram of three experiments with C, T, and DHT groups and only one for E₂ group is shown. (B) PhosphorImage imagequant quantification of Northern blots in A. Averages of three experiments are shown; bars represent the SD. The quantification was normalized according to β-actin-hybridized band intensities (as shown in A). Four different treatments were performed as described in A: control, ▪; E₂, □; T, □; DHT, □.