Figure 6.

Tissue specificity of TDD5 expression in adult male mice. C57BL/6 mice were sacrificed and several organs were collected. Northern blots were performed with the 3′ most 288 bp of TDD5 as probe. A computer-generated graph by the scanned Northern blot done with PhosphorImager is shown. (Upper) Relative intensity of TDD5-positive bands (mean ± SD) collected from three blots. The intensities of TDD5 contributed from brown fat pad (as 100) were used to normalize between blots. It should be noted that the first 17 organs, followed by hypothalamus, adrenal, and urinary bladder (Upper) correspond to the three Northern blots below (Lower). The 28S and 18S rRNAs found in total RNA preparation migrated to the positions indicated. The bands hybridized with β-actin probe for within blot normalization are shown at the bottom.