Figure 5.

Exonuclease III footprints of the top strand showing blocks around −5 and −12. Combined DNA-binding assays (A) were conducted in the presence of the 329–477 peptide (+) with either 70–324 or 180–306 peptides and compared with 329–477 peptide alone and to σ^N (1–477). Top strand contacts made by 180–477, 329–477, 365–477, and σ^N are shown in B. All proteins were used at 1 μM concentration.