Table 1.

Combinations of σ peptides that gave increased DNA footprints compared to single peptide footprints

Sigma peptides
DNA-binding		Enhancing
329–477	+	70–324
329–477	+	180–306
323–477	+	70–324
323–477	+	180–306
365–477	+	180–306

The 329–477 peptide derived from proteolysis (15) was able to substitute for the overproduced recombinant 329–477 peptide (see Figs. 2, 4, and 5, and data not shown). The 70–324 and 180–306 peptides enhance DNA binding.