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The 9,11-bridged retinal acts to reverse the effects of the Leu-93 → Ala mutant on the temperature dependence of the O intermediate. (A) Transient changes in absorbance of the Leu-93 → Ala mutant (with native retinal) measured at 600 nm to follow decay of the O intermediate. Measurements were made at 25.5, 17, and 9 ± 1°C. (B) Same as in A except native retinal was replaced by the 9,11-bridged retinal. Measurements were made at 25.5, 17, and 9 ± 1°C.
