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. 1997 May 13;94(10):5055–5060. doi: 10.1073/pnas.94.10.5055

Figure 2.

Figure 2

Karyopherin β2 binds to a fusion protein (GST-A1) containing the nuclear mRNA binding protein A1 via a specific sequence. (A) Purified recombinant karyopherin β2 analyzed by SDS/PAGE and visualized by Coomassie blue staining. (B) Immobilized GST-A1 was incubated with karyopherin β2 alone (lane 1) or with a 25× molar excess of a synthetic peptide representing the NLS of the A1 protein (lane 2) or with a 100× molar excess of the classical NLS peptide (lane 3). Bound and unbound fractions were analyzed by SDS/PAGE and Coomassie blue staining.