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Karyopherin β2 and β1 compete for binding to Nup98. Immobilized Nup98 was incubated with GST-β1 (lane 1), β2 (lane 2), GST-β1 and 10× molar excess of β2 (lane 3), or β2 and 10× molar excess of GST-β1 (lane 4). Bound and unbound fractions were analyzed by SDS/PAGE and Coomassie blue staining.
