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GST-A1 import into the nucleus requires karyopherin β2 and Ran. (A) Digitonin-permeabilized HeLa cells were incubated at 4°C with fluorescently labeled GST-A1, in the presence or absence of karyopherin β2 (2 μg/assay) as indicated. (B) Permeabilized cells were incubated at 20°C, with fluorescently labeled GST-A1 in the absence (panel 1) or in the presence of karyopherin β2 (2 μg/assay)
