Figure 4.

Signal transduction mediated by CCR5 and CCR5-GGAA in transiently transfected HEK-293T cells. (A) Inositol phosphate (IP) release from HEK-293T cells transfected with CCR5 or CCR5-GGAA in response to the indicated chemokines (100 nM). A fold increase of 1.0 corresponds to no activation. Shown is one of three similar experiments. Similar results were seen with up to 600 nM MIP-1α, MIP-1β, and RANTES (regulated on activation, normal T cell expressed and secreted). (B) Inhibition of adenylyl cyclase in transiently transfected cells was determined as described in Fig. 1. The dose-response curve was obtained with wild-type CCR5 and MIP-1β. No response was seen to the indicated concentrations of MIP-1α, MIP-1β, or RANTES in cells transfected with CCR5-GGAA. (Bars = SEM.)