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Reversible down-regulation of Bcl-xL expression upon lifting of EGF-R blockade. Bcl-xL protein expression of keratinocytes treated with control mAb 15-6A (−) and EGF-R antagonistic mAb 425 (+) for 3 days is shown in the first two lanes. Lanes 3–7 show time-dependent recovery of Bcl-xL expression levels in parallel cultures after removal of mAb 425 and addition of complete MCDB153 medium supplemented with EGF at 10 ng/ml. Representative results of one of two experiments are shown.
