Figure 1.

Mapmodulin purification. (A) Fractionation of total CHO cytosol (4 mg) by Sephacryl S100 (45 ml) gel filtration chromatography. Fractions (0.6 ml) were tested for Golgi capture activity. One unit is defined as the cpm obtained in a 60-min assay; 40 μl samples were tested. (B) Superdex S200 chromatography of Fast Flow Q fractions eluting at ≈0.5 M KCl. Fractions (1.5 ml) were collected; samples (20 μl) were analyzed by 12.5% SDS/PAGE and Coomassie blue staining. Golgi capture activity followed the polypeptide which peaked in fractions 51 and 52.