Figure 2.

Regulation of phosphorylation of PS1 CTF by various protein kinase activators and phosphoprotein phosphatase inhibitors. COS-huPS1 cells were incubated in the absence or presence of PDBu (PBu; 1 μM), calyculin A (Cal; 10 nM), cyclosporin A (Cyc; 100 nM), thapsigargin (THP; 100 nM), or forskolin (FSK; 5 μM). Cells were lysed and subjected to immunoprecipitation with α PS1-loop antiserum followed by immunoblot analysis using α PS1-loop antiserum.