Figure 3.

Effect of phosphoprotein phosphatase on electrophoretic migration of PS1 CTF. COS-huPS1 cells were treated with vehicle or PDBu, lysed, and subjected to immunoprecipitation. Sepharose-bound immune complexes were treated with λ protein phosphatase. Proteins were eluted from the Sepharose beads with Laemmli sample buffer and applied to SDS/12% PAGE, and immunoblot analysis was performed using α PS1-loop antiserum.