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Sucrose gradient fractionation of COS-huPS1 cells. Cells were treated with vehicle or PDBu and lysed using a steel-to-steel homogenizer, and extracts were applied to discontinuous sucrose gradients. Fractions were collected, and proteins were separated by SDS/12% PAGE and immunoblotted with antisera against the PS1 NTF (Ab 14) and PS1 CTF (α PS1-loop). Fractions are numbered from top to bottom of gradient.
