Figure 2.

Subcellular localization of E2F-4 and -5 is affected by coexpression of either the p107 or p130 pocket proteins or DP-2. Asynchronously growing U20S cells were transiently transfected with expression vectors encoding the proteins shown above each panel. After 40 h, overproduced E2F protein was detected in fixed cells by indirect immunofluorescence using the corresponding primary antibody and a FITC- or rhodamine-conjugated secondary antibody. In each case, location of the cotransfected protein was also determined (data not shown). Nuclei were revealed by Hoechst staining (data not shown).