Figure 4.

Effects of p107 and DP-2 on the nuclear localization of E2F-4 mutants. U2OS cells were transiently cotransfected with plasmids encoding either a mutant deficient in the ability to bind pocket proteins (E2F-4Δ18) or to bind DNA (E2F-4Δ1–81) and p107 or DP-2, as indicated above each panel (Top and Middle). E2F-4 was detected by immunofluorescence using mAb GG22 and a FITC- or rhodamine-conjugated secondary antibody. Nuclear location of coexpressed p107 or DP-2 was confirmed in each case (data not shown). (Bottom) The location of E2F-4 in U2OS cells transiently transfected with wt-E2F-4, E2F-4.NLS, or E2F-4.NES is shown.