Figure 3.

Bcl-2 channels in lipid bilayers at pH 5.4. Single-channel recordings are shown from lipid bilayers in symmetric 0.5 M KCl, 1 mM CaCl₂ adjusted to pH 5.4 after addition of 50 μg/ml His₆-Bcl-2 (ΔTM) protein. (A–C) Sample records obtained at V = 100 mV, demonstrating the occurrence of the 26 pS channels. D-F correspond to records at V = −100 mV, showing channels of 10 pS and 20 pS. Other conditions are as for Fig. 2.