Figure 5.

Effect of hGH (▪) and bGH (□) on blastocyst protein synthesis. Blastocysts were cultured for 4 hr with 0–100 ng/ml (0–4.1 nM) hGH or bGH before the incorporation of [³H]leucine into acid-precipitable protein over 2 hr was assayed as described in the text. Each point represents the mean ± SEM of three experiments, of the percentage increase from control, with each experiment including 3–9 blastocysts per treatment group (mean control values for hGH and bGH experiments are 25,314 ± 2,036 and 18,477 ± 1,020 cpm per blastocyst per 2 hr, respectively). For GH concentrations >0.01 ng/ml; P < 0.01 in comparison with 0 ng/ml GH for each set of experiments, with the exception of 100 ng/ml hGH treatment, by ANOVA.