Table 1.

GH, GH receptor, and GH-binding protein PCR primer sequences

Gene	Primer	Sequence	Nucleotides in cDNA	Fragment size, bp
GH	5′	5′-CAGCCTGATGTTTGGTACCTCGGA-3′	433–456	253
	3′	5′-GCGGCGACACTTCATGACCCGCA-3′	663–685
GH receptor	5′	5′-AGTTGGAGGAGGTGAACACCAT-3′	980–1001	330
	3′	5′-GGCACAAGAGATCAGCTTCCAT-3′	1288–1309
GH binding-protein	5′	5′-AAGTACAGCGAGTTCAGCGAAGTC-3′	739–762	146
	3′	5′-CGGATCCTCTGAAGCTGGTGATA-3′	862–884

PCR primer sequences were designed from published mouse sequences: GH (15) and GH receptor and GH-binding protein (16).