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. Author manuscript; available in PMC: 2008 Jul 14.
Published in final edited form as: J Biol Chem. 2007 May 25;282(28):20075–20087. doi: 10.1074/jbc.M700624200

FIGURE 7. Cellular location of the effect of Fyn on calpain-mediated NR2B cleavage in HEK cells.

FIGURE 7

A–C, representative blots and histogram showing NR2B levels in different cellular compartments in cells transfected with NR1/2B with or without FynY531F before (control) and 30 min after agonist application. Quantification of the NR2B levels by biotinylation showed that NR2B levels decreased to a greater degree in the lysate (A) and the surface fraction (C) in cells coexpressing FynY531F than in those expressing a vector control. In the intracellular fraction (B), quantification showed that the levels of NR2B with or without FynY531F decreased to a similar extent after agonist application. Biotinylation data were obtained from six independent experiments performed in triplicate. Error bars, S.E. wt, wild type.