Table 1.
Primers used for mutagenesis. Mutated sequences are underlined. For primers used in PCR mutagenesis procedures, only the forward primer sequence is shown.
| Primer Name | Primer Sequence | Purpose |
|---|---|---|
| HA-1 | 5’-CCGCCTTCGCAATGTACCCATACGATGTTCCAGATTACGCTTCTCAGGCATCGTCC | Introduce HA-Tag sequence |
| 71X-1 | 5’-TTGCCGGAACCCTTCTAGACCTCCCACGAAGAC | Introduce Xba I site 5’ of pp71 ORF |
| MRS | 5’-GCAGATCTTTGTCAGCCAGT | Amplify MR for random mutagenesis |
| MRAS | 5’-TGACTCGGGATGTATGCGC | Amplify MR for random mutagenesis |
| pp7162MR-For | 5’-CTCGAGTCTCTGCTGTTTTC | Introduce Xho I site for eGFP-62MR cloning |
| pp7162MR-N | 5’-CTCGAGGAACTTCGTGCCACCATGGAGGTG | N-terminal primer for 62MR-eGFP cloning |
| pp7194MR-N | 5′-CTAAACATCAAGGAGCTCACCATGGTGGGCG | N-terminal primer for 94MR-eGFP cloning |
| pp71188MR-N | 5′-GCAGAACCGAGCTCACCATGGAGG | N-terminal primer for 188MR-eGFP cloning |
| pp71MR-Rev | 5’-GTACCCGGGATGTATGCG | Introduce Xma I site for MR-eGFP cloning |
| S-tag1 | 5′-CGCCTTCGCAATGAAAGAAACCGCTGCTGCGAAATTTTCTCAGGCATCGTCC | Introduce 8 aa of the S-tag sequence |
| S-tag2 | 5′-GCTGCTAAATTTGAACGCCAGCACATGGACTCGTCTCAGGCATCGTCC | Introduce 7 aa of the S-tag sequence |
| SV40 | 5′-GATGTTCCAGATTACGCTCCAAAGAAGAAGAGAAAGGTGTCTCAGGCATCGTCCTCG | Introduce the SV40 NLS |