Table 1.
Characteristics of anti-myosin heavy chain (MHC) monoclonal antibodies (MAb) and clues for the immunohistochemical identification of the MHC isoforms expressed in skeletal muscle fibres
| MAb designation1 | |||||
|---|---|---|---|---|---|
| BF-F8 | SC-71 | BF-35 | RT-D9 | BF-F3 | |
| Class | IgG | IgG | IgG | IgM | IgM |
| Working dilution | 1: 200 | 1: 10 000 | 1: 10 000 | 1: 1000 | 1: 25 |
| Specificity2 | All except | MHC-IID/X | |||
| MHC-I | MHC-IIA | MHC-IID/X | and MHC-IIB | MHC-IIB | |
| MHC content | MAb reactivity | ||||
| I | + | − | + | − | − |
| I + IIA | + | + | + | − | − |
| IIA | − | + | + | − | − |
| IIAD/X | − | + | + | + | − |
| IID/X | − | − | − | + | − |
| IID/XB | − | − | + | + | + |
| IIB | − | − | + | + | + |
1
Source: S. Schiaffino, Padova, Italy.
2
According to Schiaffino et al. (1989). +, positive reaction for a MAb with a specific MHC-based fibre type. −, no reaction for a MAb and a specific MHC-based fibre type.