Table 3.
Primary antibodies and antisera
| Antigen | Cell type labelled | Host | Dilution | Source | Reference |
|---|---|---|---|---|---|
| Neurofilament, 200 kDa | Ganglion cell and axons; | Mouse | 1: 1000 | Chemicon International, Temecula, CA, USA (Cat. no. MAB5266; clone N52) | Shaw & Weber (1983) |
| Horizontal cell axons | |||||
| Brn-3a | Ganglion cell nuclei | Mouse | 1: 25 | Chemicon International (Cat. no. MAB1585; clone 5A3.2) | Xiang et al. (1995) |
| Microtubule associated protein-1 (MAP-1) | Retinal ganglion cells | Mouse | 1: 300 | Sigma-Aldrich, St Louis, MO, USA (Cat. no. M4278; clone HM-1) | Huber & Matus (1984) |
| Calretinin | Conventional and displaced starburst amacrine cells | Rabbit | 1: 1000 | Chemicon International (Cat. no. AB148) | Winsky et al. (1989); Pasteels et al. (1990) |
| Calbindin | Horizontal and amacrine cells | Mouse | 1: 5000 | SWANT, Bellinzona, Switzerland (Cat. no. 300) | Celio et al. (1990); Pasteels et al. (1990) |
| Choline acetyl transferase (ChAT) | Conventional and displaced starburst amacrine cells | Goat | 1: 100 | Chemicon International (Cat. no. AB144P) | Ostermann et al. (1990); Voigt (1986) |
| Protein kinase C (PKC) | Rod bipolar cells | Rabbit | 1: 100 | Calbiochem, EMD Biosciences, Inc., San Diego, USA (Cat. no. 539601) | Negishi et al. (1988); Haverkamp & Wässle (2000) |
| Vesicular glutamate transporter-1 (VGluT-1) | All photoreceptor and bipolar cell | Guinea pig terminals | 1: 1000 | Chemicon International (Cat. no. AB5905) | Johnson et al. (2003); Sherry et al. (2003a) |
| Glial fibrillary acidic protein (GFAP) | Astrocytes and reactive Müller cells | Mouse | 1: 500 | Chemicon International (Cat. no. MAB360; clone GA5) | Debus et al. (1983); Bignami & Dahl (1979); Björklund et al. (1985) |