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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Mol Cell Neurosci. 2007 Aug 23;36(4):484–500. doi: 10.1016/j.mcn.2007.08.008

Fig. 7. The knock down of gephyrin by gephyrin shRNA decreases inhibitory synaptic currents.

Fig. 7

A, Representative recordings of spontaneous synaptic responses from non-transfected (left panel), Geph CR3m-transfected (middle panel), and Geph CR-transfected neurons (right panel). Cells with typical sIPSCs are presented. B, Bar graphs showing that the average sIPSC amplitude was similar between non-transfected (112.5 ± 23.2 pA) and Geph CR3m groups (109.8 ± 19.0 pA; p>0.9). In contrast, the sIPSC amplitude in the Geph CR group was greatly decreased (56.0 ± 9.0 pA; p<0.02) comparing to the Geph CR3m group. C, Bar graphs showing that the average sIPSC frequency was similar between non-transfected (0.79 ± 0.27 Hz) and the Geph CR3m group (0.92 ± 0.22 Hz; p>0.7), but the sIPSC frequency in the Geph CR group was significantly reduced (0.16 ± 0.04 Hz; p<0.002). D, Typical recordings showing whole-cell currents induced by rapid application of GABA (20 μM) from non-transfected (left panel), Geph CR3m-transfected (middle panel), and Geph CR-transfected neurons (right panel). E, Summarized data showing no difference in the GABA current amplitude among non-transfected (2045 ± 288 pA), Geph CR3m transfected (2252 ± 202 pA; p>0.6), and Geph CR transfected neurons (1823 ± 207 pA; p>0.1).