Figure 4.

Analysis of the liver and pancreas of wild-type and double nullizygous mice. Histological analysis of liver sections from wild-type (A) and insulin-deficient mice (B). Immunocytochemical staining of pancreas sections of wild-type (C, E, and G) and insulin-deficient mice (D, F, and H) with antibodies against C-peptide 1 (C and D), C-peptide 2 (E and F), and glucagon (G and H). lacZ expression in pancreatic sections of insulin-deficient mice visualized by X-Gal staining (J) and background staining obtained with sections from wt animals (I) are shown. [Bars = 10 μm (A and B) and 8 μm (C–J).]