Table 2.

Sequence Protocol for TIPIT

Sequence	Scan Parameters	Target area
1. Midline sagittal T1 weighted Turbo Spin Echo (TSE)	TR = 337 ms, TE = 18 ms Slice thickness = 3 mm, 0.3 mm gap Scan Duration = 2 minutes	Anatomical alignment and measurement of pituitary height
2. Coronal heavily T2 weighted TSE (whole brain)	TR = 5750 ms, TE = 150 ms Slice thickness = 1.5 mm, contiguous Scan Duration = 5 minutes	Measurement of whole brain volume (cerebrum/cerebellum) and measurement of specific brain structures using stereology:
		• caudate
		• hippocampus
		• thalamus
		• prefrontal cortex
		• inferior frontal gyrus
		• temporal lobe
3. MR Angiography	TR = 25 ms, TE = 6.9 ms Slice thickness = 0.6 mm, 3D Time of Flight (TOF) Multiple Overlapping Thin Slab Acquisition (MOTSA) with a SENSE factor of 2.00. Isotropic resolution = 0.6 × 06 × 06 mm3 Scan Duration = 6 minutes	Blood vessel diameter, tortuosity, and density
4. Diffusion tensor imaging	2D single shot spin-echo Echo-Planar Imaging (SE-EPI) with SENSE factor of 2.5. 24 contiguous axial slices of 3 mm thickness with in-plane isotropic resolution of 2 mm. TR/TE = 3350/74 ms. 32 gradient directions with a b-factor of 700 s/mm2. 2 averages. Scan Duration = 3 minutes 47 s.	Measurement of degree of mean diffusivity and fractional anisotrophy (as markers of water content and myelination) in
		• posterior limb of internal capsule
		• centrum semiovale
		• anterior and posterior corpus callosum
		• frontal white matter
		• occipital white matter
5. Axial T2-weighted TSE	TR = 3023 ms, TE = 150 ms. Axial slices of 3 mm thickness with an in-plane isotropic resolution of 0.7 mm Scan Duration = 3 minutes	In same slice location as DTI to aid region drawing