Table 4.

Diversity estimate summation of side-by-side comparisons of enzyme/buffer system, cycling parameters and PCR methodology in two 'Amakihi infected with isolate K1 and KV115.

Bird ID Isolate	'Amakihi 6 K1 Kauai				'Amakihi 11 KV115 Hawaii
Enzyme	Promega			Roche	Promega			Roche
Methods	Standard	PCR+1			Standard	PCR+1
# cycles	80	80	35	35	80	80	35	35
	Trial 1	Trial 2	Trial 3	Trial 4	Trial 1	Trial 2	Trial 3	Trial 4
Haplotype 4			1
12		1
25		1					1
27		2		1
33	1
45						1
47							1
49					1	1
56							1
58		1
59								2
62			1			3
77	4	11	13	19	5	8	15	16
Haplotypes	2	5	3	2	2	4	4	2
Total # Clones	5	16	15	20	6	13	18	18
Freq. Hap 77	0.8	0.69	0.87	0.95	0.83	0.62	0.83	0.89
D (Simpson's)	1.47	2	1.32	1.1	1.38	2.25	1.42	1.24
H (S-W)	0.5	1.04	0.49	0.2	0.45	1.03	0.63	0.35
Hap/Clone Ratio	0.40	0.31	0.20	0.10	0.33	0.31	0.22	0.11