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The effect of Tyrosine-Kinase inhibition on activation of EGFR and its down-stream signaling molecules: Gefitinib inhibited the phosphorylation of EGFR and ERK and reduced Cyclin D1 expression in all cells. Total EGFR and total ERK are also shown for comparison. β-actin western blots and Coomassie blue staining of gels were used for confirmation of equal loading.
