Figure 3. PR activation turns into moesin activation.

(A), (B) (C) and (D) show the time- and dose-dependent moesin activation in T47-D breast cancer cells after recruitment of PR with P or MPA. Total cell amount of wild-type (Moesin) or Thr⁵⁵⁸-phosphorylated moesin (P-Moesin) are shown with western blot. * = P<0.05 vs control. (E) and (F) show that in MDA-MB-231 cells (that do not express PR), progesterone and MPA have no effect on moesin activation. (G) shows that moesin activation induced by progestins alone or in combination with 17β-estradiol (E2). * = P<0.05 vs control. (H) Moesin expression detected by western blot in T47-D cells transfected with moesin antisense PON for 48 h. (I) Actin remodeling after PR activation with P or MPA for 15 min in T47-D cells after transfection with moesin antisense phosphorotioate oligonucleotides (PON) (antisense - 2 µM) or sense PON (sense - 2 µM) for 48 h. Cells were stained with an Ab vs. moesin (FITC; green staining) as well as with Texas Red-phalloidin (in red). Nuclei are counterstained in blue. All the experiments were repeated three times with consistent results, and a representative result is shown.