FIG. 3.—

Sliding window analysis of human–mouse CT and GK divergence. (A) Evolutionary rates of CTs (top) and GKs (bottom) using human–mouse alignments (window size 33 codons, step size 10 codons). Red line, Ka/Ks; blue line, Ks; green line, Ka. Most regions with Ka/Ks >1.5, with P value <0.05 (bootstrap threshold computed with K-estimator 6.1). (B) Distribution of Ka, Ks, Ka/Ks in CTs and GKs using bin size 0.05 and nonparametric Kolmogorov–Smirnov test, revealing a significant difference of Ka/Ks (P = 0.0000079), thus confirming more rapid evolution of CTs than GKs.