Abstract
R64-11+ donor cells that are thermosensitive for vegetative DNA replication will synthesize DNA at the restrictive temperature when recipient minicells are present. This is conjugal DNA replication because it is R64-11 DNA that is being synthesized and there is no DNA synthesis if minicells that cannot be recipients of R64-11 DNA are used. The plasmid DNA present in the donor cells before mating is transferred to recipient minicells within the first 20 min of mating, but additional copies of plasmid DNA synthesized during the mating continue to be transferred for at least 90 min. However, the transfer of R64-11 DNA to minicells is not continuous because the plasmid DNA in minicells is the size of one R64-11 molecule or smaller, and there are delays between the rounds of plasmid transfer. DNA is synthesized in minicells during conjugation, but this DNA has a molecular weight much smaller than that of R64-11. Thus, recipient minicells are defective and are not able to complete the synthesis of a DNA strand complementary to the single-stranded R64-11 DNA received from the donor cell.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bonhoeffer F. DNA transfer and DNA synthesis during bacterial conjugation. Z Vererbungsl. 1966;98(2):141–149. doi: 10.1007/BF00897186. [DOI] [PubMed] [Google Scholar]
- Bresler S. E., Lanzov V. A., Lukjaniec-Blinkova A. A. On the mechanism of conjugation in Escherichia coli K 12. Mol Gen Genet. 1968;102(4):269–274. doi: 10.1007/BF00433718. [DOI] [PubMed] [Google Scholar]
- Brown R. D., Holt C. E. Rapid separation of thymine dimer and pyrimidines on ion-exchange paper. Anal Biochem. 1967 Aug;20(2):358–360. doi: 10.1016/0003-2697(67)90041-3. [DOI] [PubMed] [Google Scholar]
- CURTIS S. R., 3rd CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI. J Bacteriol. 1965 Jan;89:28–40. doi: 10.1128/jb.89.1.28-40.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carrier W. L., Setlow R. B. Paper strip method for assaying gradient fractions containing radioactive macromolecules. Anal Biochem. 1971 Oct;43(2):427–432. doi: 10.1016/0003-2697(71)90272-7. [DOI] [PubMed] [Google Scholar]
- Cohen A., Fisher W. D., Curtiss R., 3rd, Adler H. I. DNA isolated from Escherichia coli minicells mated with F+ cells. Proc Natl Acad Sci U S A. 1968 Sep;61(1):61–68. doi: 10.1073/pnas.61.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen A., Fisher W. D., Curtiss R., 3rd, Adler H. I. The properties of DNA transferred to minicells during conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:635–641. doi: 10.1101/sqb.1968.033.01.071. [DOI] [PubMed] [Google Scholar]
- Cooke M., Meynell E., Lawn A. M. Mutant Hfr strains defective in transfer: restoration by F-like and I-like de-repressed R factors. Genet Res. 1970 Aug;16(1):101–112. doi: 10.1017/s0016672300002317. [DOI] [PubMed] [Google Scholar]
- Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. doi: 10.1146/annurev.mi.23.100169.000441. [DOI] [PubMed] [Google Scholar]
- Fenwick R. G., Jr, Curtiss R., 3rd Conjugal deoxyribonucleic acid replication by Escherichia coli K-12: effect of nalidixic acid. J Bacteriol. 1973 Dec;116(3):1236–1246. doi: 10.1128/jb.116.3.1236-1246.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fenwick R. G., Jr, Curtiss R., 3rd Conjugal deoxyribonucleic acid replication by Excherichia coli K-12: effect of chloramphenicol and rifampin. J Bacteriol. 1973 Dec;116(3):1224–1235. doi: 10.1128/jb.116.3.1224-1235.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freifelder D., Folkmanis A., Kirschner I. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J Bacteriol. 1971 Mar;105(3):722–727. doi: 10.1128/jb.105.3.722-727.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulton C. Continuous chromosome transfer in Escherichia coli. Genetics. 1965 Jul;52(1):55–74. doi: 10.1093/genetics/52.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
- Inselburg J. Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. J Bacteriol. 1970 Jun;102(3):642–647. doi: 10.1128/jb.102.3.642-647.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kass L. R., Yarmolinsky M. B. Segregation of functional sex factor into minicells. Proc Natl Acad Sci U S A. 1970 Jul;66(3):815–822. doi: 10.1073/pnas.66.3.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
- Levy S. B., Norman P. Segregation of transferable R factors into Escherichia coli minicells. Nature. 1970 Aug 8;227(5258):606–607. doi: 10.1038/227606a0. [DOI] [PubMed] [Google Scholar]
- Marinus M. G., Adelberg E. A. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. J Bacteriol. 1970 Dec;104(3):1266–1272. doi: 10.1128/jb.104.3.1266-1272.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsubara K. Properties of sex factor and related episomes isolated from purified Escherichia coli zygote cells. J Mol Biol. 1968 Nov 28;38(1):89–108. doi: 10.1016/0022-2836(68)90130-7. [DOI] [PubMed] [Google Scholar]
- McGrath R. A., Williams R. W. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature. 1966 Oct 29;212(5061):534–535. doi: 10.1038/212534a0. [DOI] [PubMed] [Google Scholar]
- Meynell E., Meynell G. G., Datta N. Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968 Mar;32(1):55–83. doi: 10.1128/br.32.1.55-83.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
- Paterson M. C., Roozen K. J. Photoreactivatiion, excision, and strand-rejoining repair in R factor-containing minicells of Escherichia coli K-12. J Bacteriol. 1972 Apr;110(1):71–80. doi: 10.1128/jb.110.1.71-80.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Regan J. D., Setlow R. B., Ley R. D. Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine. Proc Natl Acad Sci U S A. 1971 Apr;68(4):708–712. doi: 10.1073/pnas.68.4.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roozen K. J., Fenwick R. G., Jr, Curtiss R., 3rd Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12. J Bacteriol. 1971 Jul;107(1):21–33. doi: 10.1128/jb.107.1.21-33.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rupp W. D., Ihler G. Strand selection during bacterial mating. Cold Spring Harb Symp Quant Biol. 1968;33:647–650. doi: 10.1101/sqb.1968.033.01.073. [DOI] [PubMed] [Google Scholar]
- Sheehy R. J., Orr C., Curtiss R., 3rd Molecular studies on entry exclusion in Escherichia coli minicells. J Bacteriol. 1972 Nov;112(2):861–869. doi: 10.1128/jb.112.2.861-869.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stallions D. R., Curtiss R., 3rd Chromosome transfer and recombinant formation with deoxyribonucleic acid temperature-sensitive strains of Escherichia coli. J Bacteriol. 1971 Mar;105(3):886–895. doi: 10.1128/jb.105.3.886-895.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vapnek D., Lipman M. B., Rupp W. D. Physical properties and mechanism of transfer of R factors in Escherichia coli. J Bacteriol. 1971 Oct;108(1):508–514. doi: 10.1128/jb.108.1.508-514.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vapnek D., Rupp W. D. Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli. J Mol Biol. 1970 Nov 14;53(3):287–303. doi: 10.1016/0022-2836(70)90066-5. [DOI] [PubMed] [Google Scholar]
- Vapnek D., Rupp W. D. Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli. J Mol Biol. 1971 Sep 28;60(3):413–424. doi: 10.1016/0022-2836(71)90178-1. [DOI] [PubMed] [Google Scholar]
- Willetts N. S. Plasmid specificity of two proteins required for conjugation in E. coli K12. Nat New Biol. 1971 Apr 7;230(14):183–185. doi: 10.1038/newbio230183a0. [DOI] [PubMed] [Google Scholar]
- Willetts N. S. The interaction of an I-like R factor and transfer-deficient mutants of Flac in E. coli K 12. Mol Gen Genet. 1970;108(4):365–373. doi: 10.1007/BF00267774. [DOI] [PubMed] [Google Scholar]