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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: Vaccine. 2008 Feb 20;26(16):2020–2033. doi: 10.1016/j.vaccine.2008.02.009

Table 3.

Effect of vaccines on IC challenge with WNV in wild type mice

Vaccination Challenge Dose WNV PFU # survived/total P value
Challenge: Day 60, no boost
Mock, no challenge 0 10/10
Placebo 101 0/10
DNA plasmid 101 4/10 0.04
Inactivated WNV 101 7/10 0.001
Live WNV (survivors) 101 10/10 < 0.0001
Placebo 103 0/10
DNA plasmid 103 3/10 0.1
Inactivated WNV 103 8/10 0.0004
Live WNV (survivors) 103 9/10 < 0.0001
Challenge: Day 70, one boost
Mock 101 0/10
Placebo 101 1/10
DNA plasmid 101 4/10 0.4
Inactivated WNV 101 9/10 < 0.0001
Live WNV (survivors) 101 10/10 < 0.0001

Wild type mice were vaccinated DNA plasmid (10 μg), formalin-inactivated WNV New York (100 μl), or placebo vaccines. Mice were challenge after primary immunization at day 60 or ten days after a boost, at day 70 with either 101 or 103 PFU of WNV by an IC route and monitored for survival for 28 days. Survival was compared to re-challenge of the subset of animals surviving primary infection with live virulent WNV New York. P values were determined using the log rank test.