. Author manuscript; available in PMC: 2008 Jul 15.

Published in final edited form as: Biochemistry. 2006 Jan 17;45(2):604–616. doi: 10.1021/bi0517178

Table 2.

vant Hoff Measurements of ΔH and T_M for Single Strand Melting of Component Strands^a

	0.1 M NaCl		1.0 M NaCl
strand	ΔH^vH (kcal mol⁻¹)	T_M(°C)	ΔH^vH (kcal mol⁻¹)	T_M(°C)
D-I S1	36.5 ± 0.7	9.0 ± 0.4	40.1 ± 0.7	25.7 ± 0.3
D-I S2	23.4 ± 0.5	34.9 ± 0.2	21 ± 2	45.6 ± 0.9
D-II S1	31.0 ± 0.5	14.8 ± 0.3	29 ± 1	28.8 ± 0.5
D-II S2	24.8 ± 0.2	44.5 ± 0.2	31 ± 1	44.1 ± 0.3
D-III S1	35 ± 5	10 ± 2	30 ± 2	26 ± 1
D-III S2	26.0 ± 0.2	39.6 ± 0.1	27.0 ± 0.7	44.0 ± 0.2
D-IV S1	ND^b	ND^b	30 ± 4	42 ± 1
D-IV S2	18.6 ± 0.5	37.5 ± 0.3	24 ± 1	43.5 ± 0.4
D-V S1	19 ± 5	27 ± 3	18 ± 2	32 ± 3
D-V S2	28.9 ± 0.7	41.0 ± 0.3	25 ± 1	46.7 ± 0.3

Parameters derive from direct curve fitting of optical melting profiles. All samples contained 2.5 μM strand, 10 mM NaHEPES, pH 7.5, and 0.1 or 1.0 M added NaCl.

ND: not determined due to the small Δє and low temperature of the single strand melting transition.