Figure 1.

Maps of the vectors GMRE and GMREP. The positions of the white marker gene (white), sequences for plasmid rescue (amp and ori), and the pentamer of GLASS binding sites (GBS) are indicated. Restriction endonuclease sites for plasmid rescue are represented by short vertical lines. Primer sequences used for PCR are indicated by small arrowheads (GMR P1 and GMR P 3′). In GMREP, the TATA box and 5′ splice donor sequences are indicated. The Bluescript polylinker (BS) is indicated as a triangle with vertical lines.