Table 2.
Effect of caf20::TRP1 on growth rates
| Strain (relevant genotype) | Doubling time, h
|
||
|---|---|---|---|
| 30°C | 33°C or 35°C | 18°C | |
| Wild type | 2.0 | 1.9 | 5.7 |
| caf20::TRP1 | 2.0 | 1.9 | 5.6 |
| stm1::ADE2 | 3.7 | 4.5 | 14.1 |
| stm1::ADE2 caf20::TRP1 | 3.0 | 3.6 | 8.3 |
| tif4631::LEU2 | 2.9 | 2.9 | 10.2 |
| tif4631::LEU2 caf20::TRP1 | 2.5 | 2.4 | 8.0 |
| cdc33-1 | 4.5 | >20* | ND |
| cdc33-1 caf20::TRP1 | 4.0 | >20* | ND |
| cdc33-42 | 3.0 | 8.1* | ND |
| cdc33-42 caf20::TRP1 | 2.7 | 7.2* | ND |
| tif1-1 | 3.2 | 5.0* | ND |
| tif1-1 caf20::TRP1 | 3.0 | 4.8* | ND |
| prt1-1 | 4.0 | >20* | ND |
| prt1-1 caf20::TRP1 | 3.5 | >20* | ND |
| ded1/spp81-3 | 4.5 | ND | ND |
| ded1/spp81-3 caf20::TRP1 | 3.7 | ND | ND |
Cultures were grown in yeast peptone dextrose medium at the indicated temperatures and growth measured at OD600. Data are the average of at least three different experiments. Standard deviation was less than 0.3 h. ND, not determined.
*
Growth at 33°C.