Fig. 3.

FVB mice failed to reject ANV^pos despite the presence of neu-specific immune responses. a Levels of neu expression in three mammary tumor lines used in this study were determined by flow cytometry. Autofluorescence and control Abs showed similar background fluorescence. Data are representative of three independent experiments; b FVB mice (n = 4) were inoculated with ANV, ANV^pos, or MMC (5 × 10⁶ cells/mouse) and tumor growth was monitored once every 3 days; c Sera were collected from the FVB mice 3 weeks after the tumor challenge and subjected to ELISA using plates coated with the ECD of rat neu protein (1 μg/well). Sera from naïve mice were used as negative controls. Data are presented as mean Ab titers; d Above-mentioned groups were sacrificed 7 weeks after the tumor challenge and their splenocytes were subjected to IFN-γELISA