Fig. 1.

FcRγ^−/− mouse: histology. FcRγ^−/− mice underwent 7 days of I/RC with or without SAP administration. (A–C) Tissue sections were stained for collagen (graph: n = 5/6 per group) (A), α-SMA (n = 7 per group) (B), and macrophages (n = 8/9 per group) (C). (Magnification: ×100.) Ischemic and nonischemic (posterior septum) regions were analyzed; the latter served as an interior control because this region should not be affected by I/RC. In contrast to wild-type mice (6), FcRγ^−/− mice were not protected by SAP. SAP did not inhibit the interstitial deposition of collagen and did not diminish the amount of α-SMA⁺ cells. However, consistent with our previous observation (6), SAP during I/RC given to either wild-type or FcRγ^−/− mice did not reduce the number of macrophages. In sham-treated FcRγ^−/− mice (with and without SAP treatment), no differences between ischemic and nonischemic regions were observed for collagen, α-SMA⁺ cells, and macrophages (data not shown). *, P < 0.05 between ischemic and nonischemic groups; NS, not significant.