Figure 2.

Schematic representation of MCD mapping process. (a) Gel image. (b) List of fragment sizes for each enzyme domain in each clone. Lanes labeled with a number identify the clone as c01 or c02. Lanes labeled with the letter M identify size markers. (c) Three single-enzyme maps are independently constructed (Right). Synchronization across enzyme domains results in a composite map (Left). Long tick marks indicate boundaries between ordered groups of fragments; short tick marks demarcate unordered fragments within a group, arbitrarily drawn in order of decreasing size.