Figure 5.

Representative MCD map from chromosome 7. Four hybrid cell-line-derived YACs were subcloned into cosmids to map this 400 kbp region. In addition, a special cosmid library derived directly from the hybrid cell line (i.e., not derived from a YAC clone) was also placed on this map, with no inconsistencies. The map is depicted just below the upper scale bar. Enzyme domains EcoRI, HindIII, and NsiI are depicted, from top to bottom, in red, green, and blue. Ordered groups of fragments are separated by tall tick marks and unordered fragments within a group are separated by short tick marks. The minimal-tiling-path clones are displayed in purple just below the map. Below the tiling path clones, a larger set of clones is shown: this set includes all clones except those whose fragment content is identical to, or a subset of, that of a displayed clone. Next is a series of five histograms. From top to bottom, they reflect cosmid coverage derived from the following sources: the cosmid library prepared directly from hybrid cell line DNA, yWSS1613, yWSS771, yWSS1572, and yWSS1434. Below the histograms is a map quality assessment based on atlas (E. Thayer, unpublished work).